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Record W2431732493 · doi:10.1007/978-1-61737-988-8_3

Canola Zygotic Embryo Culture

2010· article· en· W2431732493 on OpenAlexaff
Nicole S. Ramesar-Fortner, Edward C. Yeung

Bibliographic record

VenueMethods in molecular biology · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant tissue culture and regeneration
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsEmbryoZygoteBiologySomatic embryogenesisMaternal to zygotic transitionEmbryogenesisCanolaEmbryo cultureCell biologyBotany

Abstract

fetched live from OpenAlex

To further understand the events occurring during embryogenesis, it is imperative to have an experimental system. While somatic embryo is the system of choice due to the prolific number and success of protocols available, it is necessary to compare in vitro results with those in vivo. This process is often difficult due to the inaccessibility of the developing embryo and the complications of manipulating the embryo in vivo. The development of protocols that allow for manipulation and comparison of both somatic and zygotic embryos is key to elucidating the differences between the two types of embryos and determining if results observed using one system can be applied to the other. This chapter details a simple protocol for the culture of zygotic embryos of canola that allows for the processing of large numbers of embryos with little physical damage. Furthermore, this protocol allows for the experimental manipulation of zygotic embryos in vitro and comparisons to be made with a well-established microspore-derived embryo system.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.012
Threshold uncertainty score0.039

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0020.002
Science and technology studies0.0020.001
Scholarly communication0.0020.001
Open science0.0030.002
Research integrity0.0010.003
Insufficient payload (model declined to judge)0.0120.013

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.351
Teacher spread0.339 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes1
Has abstractyes

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