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Record W2432195883 · doi:10.1385/1-59259-184-1:183

Proteolytic Fragments of Calcium-Binding Proteins

2003· article· en· W2432195883 on OpenAlexaff
Richard D. Brokx, Hans J. Vogel

Bibliographic record

VenueHumana Press eBooks · 2003
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSignaling Pathways in Disease
Canadian institutionsUniversity of CalgaryUniversity of Toronto
Fundersnot available
KeywordsCalmodulinHelix (gastropod)Calcium-binding proteinChemistryEF handProteolysisPeptideProtein structureBiochemistryBiophysicsStereochemistryCrystallographyCalciumBiology

Abstract

fetched live from OpenAlex

A major family of Ca2+-binding proteins is the “EF-hand” superfamily (1), so-called because they all contain “EF-hand” helix-loop-helix Ca2+-binding motifs. These motifs predominantly exist in pairs in these proteins, which is important for high-affinity, cooperative binding of Ca2+ions. Separation of individual helix-loop-helix domains through proteolysis provides an ideal starting point to asses the importance of having these domains in pairs. In addition to proteolytic methods, helix-loop-helix domains can be synthesized chemically through solid-phase peptide synthesis (2). This permits total freedom in choosing mutation sites and the location of the start and end of the polypeptide chains, but can be cost-prohibitive because of the size (approx 35 residues) of the peptides needed. Whatever the route they obtain, isolated EF-hands are interesting models of Ca2+-binding proteins. Often, these isolated motifs, such as thrombic fragements of calmodulin (CaM) (3) and synthetic troponin-C peptides (2,4,5,4,5) dimerize in vitro to form native-like structures.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.038
Threshold uncertainty score0.573

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.058
GPT teacher head0.289
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2003
Admission routes1
Has abstractyes

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