[Preparation of monoclonal antibody against bovine phosphoenolpyruvate carboxykinase].
Bibliographic record
Abstract
OBJECTIVE: To prepare the monoclonal antibody against bovine phosphoenolpyruvate carboxykinase (PEPCK) and characterize its biological functions. METHODS: The recombinant plasmid containing PEPCK gene was constructed and used to transfect Escherichia coli. After expression induction in E.coli, the recombinant protein PEPCK was purified and used to immunize BALB/c mice. After the spleen B cells in the immunized mice were fused with murine myeloma cells, the positive clones were identified and selected by indirect ELISA for titer determination. PEPCK mAb produced by the positive hybridoma cells was enriched and its biological functions were examined by Western blotting, immunohistochemistry and immunoprecipitation. RESULTS: One hybridoma cell line steadily secreting PEPCK mAb was successfully generated, namely 3D36H. Western blotting, immunohistochemistry and immunoprecipitation showed that the PEPCK mAb was able to specifically bind to bovine PEPCK protein. CONCLUSION: The bovine PEPCK mAb was prepared successfully and had a good ability and specificity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.014 | 0.010 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".