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Record W2437388877 · doi:10.1038/ncomms10476

Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

2016· article· en· W2437388877 on OpenAlexafffund
Emiley A. Eloe‐Fadrosh, David Páez-Espino, Jessica K. Jarett, Peter F. Dunfield, Brian P. Hedlund, Anne Dekas, Stephen E. Grasby, Allyson L. Brady, Hailiang Dong, Brandon R. Briggs, Wen‐Jun Li, Danielle Goudeau, Rex R. Malmstrom, Amrita Pati, Jennifer Pett‐Ridge, Edward M. Rubin, Tanja Woyke, Nikos C. Kyrpides, Natalia Ivanova

Bibliographic record

VenueNature Communications · 2016
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsMcMaster UniversityGeological Survey of CanadaUniversity of Calgary
FundersLawrence Livermore National LaboratoryOffice of International Science and EngineeringOffice of ScienceNational Aeronautics and Space AdministrationGenome British ColumbiaUniversity of Nevada, Las VegasMinistry of Science and Technology of the People's Republic of ChinaNational Energy Research Scientific Computing CenterGenome CanadaJoint Genome InstituteU.S. Department of EnergyGovernment of AlbertaGenome AlbertaNational Science Foundation
KeywordsMetagenomicsPhylumBiologyLineage (genetic)Evolutionary biologyGenomeComputational biologyGenomicsTree of life (biology)Ribosomal RNAPhylogeneticsPhylogenomicsGeneGeneticsClade

Abstract

fetched live from OpenAlex

Analysis of the increasing wealth of metagenomic data collected from diverse environments can lead to the discovery of novel branches on the tree of life. Here we analyse 5.2 Tb of metagenomic data collected globally to discover a novel bacterial phylum ('Candidatus Kryptonia') found exclusively in high-temperature pH-neutral geothermal springs. This lineage had remained hidden as a taxonomic 'blind spot' because of mismatches in the primers commonly used for ribosomal gene surveys. Genome reconstruction from metagenomic data combined with single-cell genomics results in several high-quality genomes representing four genera from the new phylum. Metabolic reconstruction indicates a heterotrophic lifestyle with conspicuous nutritional deficiencies, suggesting the need for metabolic complementarity with other microbes. Co-occurrence patterns identifies a number of putative partners, including an uncultured Armatimonadetes lineage. The discovery of Kryptonia within previously studied geothermal springs underscores the importance of globally sampled metagenomic data in detection of microbial novelty, and highlights the extraordinary diversity of microbial life still awaiting discovery.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.281
Teacher spread0.264 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations154
Published2016
Admission routes2
Has abstractyes

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