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Record W2465199382 · doi:10.1385/1-59259-294-5:263

Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity

2003· article· en· W2465199382 on OpenAlexaff
Warren W. Wakarchuk, Anna‐Maria Cunningham

Bibliographic record

VenueHumana Press eBooks · 2003
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGlycosylation and Glycoproteins Research
Canadian institutionsInstitute for Biological Sciences
Fundersnot available
KeywordsGlycobiologyGlycosyltransferaseBiochemistryCapillary electrophoresisChemistryComputational biologyCloning (programming)EnzymeBiologyGlycoproteinChromatographyGlycanComputer science

Abstract

fetched live from OpenAlex

Glycobiology has rapidly developed into an important area of research devoted to the understanding and analysis of the interactions that carbohydrates have with other biomolecules. To accompany the rapid development of this field, better techniques for the analysis of the biosynthesis of complex carbohydrates are required. To investigate interactions between proteins and carbohydrates, an adequate supply of carbohydrate ligand must be obtained. Isolation of biologically active carbohydrates is possible, but only in small amounts. Recent efforts in the cloning of glycosyltransferase genes from a variety of sources has opened the possibility of enzymatic synthesis or chemienzymatic synthesis of a larger number of relevant oligosaccharides (1–4, ).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.151
Threshold uncertainty score0.831

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.354
Teacher spread0.308 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations25
Published2003
Admission routes1
Has abstractyes

Explore more

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