In vivo Effects of Intraperitoneally Injected ʟ-AsparaginaseSolution and ʟ-Asparaginase Immobilized within SemipermeableNylon Microcapsules with Emphasis on Blood ʟ-Asparaginase,‘Body’ ʟ-Asparaginase, and Plasma ʟ-Asparagine Levels
Bibliographic record
Abstract
C3H/HEJ mice were given i.p. injections of one of the following: ʟ-asparaginase solution, microencapsulated ʟ-asparaginase, saline or control microcapsules. After injection of ʟ-asparaginase solution, enzyme activity appeared in the blood very rapidly with the highest concentration occurring after 4 h, and was then cleared from the circulation with a half-life of 4.4 h. In marked contrast, when microencapsulated ʟ-asparaginase was injected, no significant ʟ-asparaginase activity appeared in the blood for the entire duration of this study. ‘Body’ ʟ-asparaginase levels declined very rapidly with a half-life of 2 h after injection of ʟ-asparaginase solution, whereas it took 60-72 h for the ‘body' ʟ-asparaginase to decrease to 50% of the original activity after injection of microencapsulated ʟ-asparaginase. The microencapsulated ʟ-asparaginase still retained about 20% of its original activity up to 16 days after injection. Plasma ʟ-asparagine was maintained at zero concentration for 3 days after injection of ʟ-asparaginase solution, compared to 8 days after injection for microencapsulated ʟ-asparaginase. Liver ʟ-asparaginase activity was found to increase after injection of ʟ-asparaginase solution but not after injection of microencapsulated ʟ-asparaginase. The response of the host to i.p. injection of nylon microcapsules is described. Preliminary experiments indicate that the half-life for clearance of the free enzyme from the circulation of 6C3HED lymphosarcoma-bearing mice was 13.2 h as compared to 4.4 h for normal mice, and that microencapsulated ʟ-asparaginase was capable of causing regression of the tumor in the advanced, well-established stage.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".