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Record W2477184618 · doi:10.1385/0-89603-076-8:373

Autoradiographic Methods for the Localization of Amine Receptor Sites in Neural Tissue

2003· book-chapter· en· W2477184618 on OpenAlexaff
R.A. Leslie, Chad A. Shaw, H.A. Robertson, Kathryn M. Murphy

Bibliographic record

VenueHumana Press eBooks · 2003
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicReceptor Mechanisms and Signaling
Canadian institutionsDalhousie University
Fundersnot available
KeywordsReceptorNeurotransmitter receptorLigand (biochemistry)NeurotransmitterBiochemistryChemistryMembraneIn vitroBiologyBiophysicsCell biology

Abstract

fetched live from OpenAlex

Many of the most significant advances in neurobiology in the 1970s relate to the study of receptor function Receptors are proteinaceous membrane components that, when occupied by a specific ligand (neurotransmitter, neuromodulator, or hormone), will initiate a cellular response The most important techniques that have been developed recently to advance such studies involve ways of measuring directly the interactions between a neurotransmitter or drug and its receptor. With few exceptions, these procedures involve an in vitro technique in which animals are sacrificed, their brains removed and dissected into various specific regions according to some standardized procedure, and the dissected regions homogenized and centrifuged to yield a membrane preparation that includes the receptors of interest. Sometimes crude synaptosomal (P2) pellets are used in the final binding assay that follows these procedures, but more often homogenates are the source of receptor material. Aliquots of the homogenate are then incubated with various concentrations of a radioactive ligand, specific for the receptors of interest, in the presence or the absence of displacing concentrations of a “cold” (nonradioactive) ligand, often called a displacer

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: none
Teacher disagreement score0.033
Threshold uncertainty score0.111

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0050.002
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0020.001
Research integrity0.0010.004
Insufficient payload (model declined to judge)0.0330.021

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.053
GPT teacher head0.323
Teacher spread0.269 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2003
Admission routes1
Has abstractyes

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