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Record W2483133705 · doi:10.1007/978-1-59745-198-7_46

Zn2+-Reverse Staining Technique

2009· book-chapter· en· W2483133705 on OpenAlex
Carlos Fernández-Patrón

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueSpringer protocols handbooks/Springer protocols · 2009
Typebook-chapter
Languageen
FieldChemistry
TopicAdvanced Proteomics Techniques and Applications
Canadian institutionsUniversity of AlbertaCanadian Institutes of Health ResearchHeart and Stroke Foundation
FundersCanadian Institutes of Health ResearchHeart and Stroke Foundation of Canada
KeywordsStainingPosttranslational modificationChemistryProtein detectionProteomicsMolecular biologyComputational biologyBiochemistryBiologyNanotechnologyMaterials scienceGeneEnzyme

Abstract

fetched live from OpenAlex

With the advent of “proteomics,” many previously unidentified proteins will be isolated in gels for subsequent structural and functional characterization. It is, therefore, important that methods are available for detecting these proteins with minimal risk of modification. This chapter describes a “reverse” staining technique that facilitates the sensitive detection of unmodified proteins (1–9).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity, Insufficient payload (model declined to judge)
Consensus categoriesMeta-epidemiology (narrow), Research integrity
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Protocol · Consensus signal: Protocol
Teacher disagreement score0.660
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0020.003
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0010.000
Science and technology studies0.0010.001
Scholarly communication0.0010.000
Open science0.0030.001
Research integrity0.0020.004
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.323
Teacher spread0.287 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it