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Record W2497813984 · doi:10.1385/1-59259-207-4:307

Colony Cultures: Plating Efficiency Assay and Cloning

2003· book-chapter· en· W2497813984 on OpenAlexaff
S. Fedoroff, Arleen Richardson

Bibliographic record

VenueHumana Press eBooks · 2003
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicViral Infectious Diseases and Gene Expression in Insects
Canadian institutionsUniversity of Saskatchewan
Fundersnot available
Keywordsclone (Java method)BiologyCloning (programming)Cell cultureSuspension cultureCell biologyPlating efficiencyMorphology (biology)Colony morphologyGeneticsDNABacteriaComputer science

Abstract

fetched live from OpenAlex

The premise of colony culturing is the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony or clone. Colony cultures can be initiated either from a disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures or cell lines. Colonies, especially ones initiated directly from tissues, are not all identical. They vary in the morphology of their cells, and in their size and compactness. The morphology and size of the colony depends on the kinds of cells plated, interactions between cells, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions to which the cells are subjected.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.015
Threshold uncertainty score0.049

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.004
Meta-epidemiology (narrow)0.0030.002
Meta-epidemiology (broad)0.0040.002
Bibliometrics0.0090.008
Science and technology studies0.0010.001
Scholarly communication0.0020.001
Open science0.0050.002
Research integrity0.0020.004
Insufficient payload (model declined to judge)0.0150.029

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.270
Teacher spread0.246 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2003
Admission routes1
Has abstractyes

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