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Record W2512549315 · doi:10.1002/eji.201646406

NR4A1‐dependent Ly6C<sup>low</sup> monocytes contribute to reducing joint inflammation in arthritic mice through Treg cells

2016· article· en· W2512549315 on OpenAlex
Alexandre Brunet, Manon Lebel, Benoit Egarnes, Carine Paquet‐Bouchard, Anne‐Julie Lessard, Jacques P. Brown, Jean Gosselin

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueEuropean Journal of Immunology · 2016
Typearticle
Languageen
FieldNeuroscience
TopicNuclear Receptors and Signaling
Canadian institutionsCentre hospitalier de l'Université LavalUniversité Laval
FundersInstitute of Musculoskeletal Health and ArthritisCanadian Institutes of Health ResearchArthritis Society
KeywordsImmunologyInflammationFOXP3ArthritisAdoptive cell transferMonocyteIL-2 receptorMedicineBiologyT cellImmune system

Abstract

fetched live from OpenAlex

Monocytes are central to the physiopathology of arthritis, but their roles in progression and resolution of the disease remain to be clarified. Using NR4A1 −/− mice, which lack patrolling lymphocyte antigen 6C (Ly6C low ) monocytes, we found that inflammatory Ly6C high monocytes contribute to rapid development of arthritis in a serum transfer‐induced arthritis (STIA) model. Our experiments suggest that patrolling monocytes do not promote the initiation and progression of arthritis in mice, as severity of symptoms was amplified in NR4A1 −/− mice. Moreover, we show that treatment of arthritic wild type (WT) mice with cytosporone B (Csn‐B), a NR4A1‐specific agonist, significantly reduces severity of disease. Effects of Csn‐B were absent in monocyte‐depleted mice treated with clodronate until Ly6C low monocytes were restored. Adoptive transfer of Ly6C low monocytes in arthritic NR4A1 −/− mice treated with Csn‐B reduces joint inflammation, supporting the regulatory role of Ly6C low subset on disease development. Our results also reveal that administration of Csn‐B to arthritic mice enhances levels of circulating CD4 + CD25 + FoxP3 + Treg cells, a process requiring the presence of Ly6C low monocytes. Together, these data indicate that Ly6C high monocytes are involved in the initiation and progression of arthritis and Ly6C low monocytes contribute to reduce joint inflammation through the mobilization of Treg cells.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.022
Threshold uncertainty score0.773

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.231
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it