Bibliographic record
Abstract
WRKY类转录因子是植物防御反应信号转导的重要组成部分,本研究为了探讨WRKY33基因的功能,以嫩叶为材料,采用RT-PCR和RACE技术相结合的方法,克隆得到WRKY33全长c DNA(命名为Ps WRKY33),对其进行生物信息学分析。以基因组DNA为模板,对该基因的ORF区域进行PCR扩增,获得WRKY33的g DNA全长。采用荧光定量PCR技术检测该基因在水杨酸处理下的表达模式。试验结果表明:Ps WRKY33 c DNA全长为2 113 bp,开放阅读框长为1 770 bp,编码589个氨基酸;Ps WRKY33蛋白含有两个WRKY保守结构域,与Pm WRKY33蛋白同源性最高。Ps WRKY33基因组全长为2 844 bp,存在3个内含子和4个外显子。荧光定量结果表明:Ps WRKY33在1 mmol/L水杨酸处理后,30 h表达量最高,呈先上升后下降的趋势。研究结果为深入研究WRKY33基因与水杨酸诱导植物抗病性的关系提供一定的参考价值。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.007 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".