Synthetic seed production for germplasm storage of Hydrastis canadensis L. (Goldenseal)
Bibliographic record
Abstract
Goldenseal (Hydrastis canadensis L.) is an important medicinal plant, native to Canada and Eastern United States, used as an antibiotic, anticonvulsant and in treatments for inflammations and dyspepsia. Over collection of wild population and native habitat destruction has lead the species to be listed as an endangered species in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), Appendix II. This brought a revived interest in conservation of germplasm.;Calcium alginate solid and hollow bead encapsulation protocols were evaluated. Shoot and callus propagules were encapsulated in calcium alginate solid bead (supplemented with stage II nutrient media) and hollow bead (no nutrient supplements) and were stored at 25°C and 5°C for either 4, 8 or 12 weeks. Best regeneration and viability were observed in 2.5% alginate concentrated solid shoot propagules that were stored at 5°C. By the end of 6 weeks of sub culturing 100% regeneration was achieved. Reduced (10%) regeneration was observed in callus propagules stored at 5°C, and all the callus propagules stored at 25°C were necrotic.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".