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Record W2526418622 · doi:10.1371/journal.pone.0162624

Towards a DNA Barcode Reference Database for Spiders and Harvestmen of Germany

2016· article· en· W2526418622 on OpenAlexfundno aff
Jonas J. Astrin, Hubert Höfer, Jörg Spelda, Joachim Holstein, Steffen Bayer, Lars Hendrich, Bernhard A. Huber, Karl-Hinrich Kielhorn, Hans‐Joachim Krammer, Martin Lemke, Juan Monje, Jérôme Morinière, Björn Rulik, Malte Petersen, Hannah Janssen, Christoph Muster

Bibliographic record

VenuePLoS ONE · 2016
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSpider Taxonomy and Behavior Studies
Canadian institutionsnot available
FundersOntario Ministry of Research and InnovationNatural Sciences and Engineering Research Council of CanadaBayerisches Staatsministerium für Bildung und Kultus, Wissenschaft und KunstGovernment of CanadaGenome CanadaOntario GenomicsBundesministerium für Bildung und ForschungOntario Genomics Institute
KeywordsDNA barcodingIntraspecific competitionOpilionesBiologyFaunaBarcodeSpiderInterspecific competitionZoologyEcologyEvolutionary biology

Abstract

fetched live from OpenAlex

As part of the German Barcode of Life campaign, over 3500 arachnid specimens have been collected and analyzed: ca. 3300 Araneae and 200 Opiliones, belonging to almost 600 species (median: 4 individuals/species). This covers about 60% of the spider fauna and more than 70% of the harvestmen fauna recorded for Germany. The overwhelming majority of species could be readily identified through DNA barcoding: median distances between closest species lay around 9% in spiders and 13% in harvestmen, while in 95% of the cases, intraspecific distances were below 2.5% and 8% respectively, with intraspecific medians at 0.3% and 0.2%. However, almost 20 spider species, most notably in the family Lycosidae, could not be separated through DNA barcoding (although many of them present discrete morphological differences). Conspicuously high interspecific distances were found in even more cases, hinting at cryptic species in some instances. A new program is presented: DiStats calculates the statistics needed to meet DNA barcode release criteria. Furthermore, new generic COI primers useful for a wide range of taxa (also other than arachnids) are introduced.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.007
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.012
Threshold uncertainty score0.030

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.007
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0120.007
Science and technology studies0.0010.000
Scholarly communication0.0020.002
Open science0.0020.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0090.014

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.088
GPT teacher head0.272
Teacher spread0.184 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations142
Published2016
Admission routes1
Has abstractyes

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Same venuePLoS ONESame topicSpider Taxonomy and Behavior StudiesFrench-language works237,207