CASPASE-9 AND CASPASE-3/7 ACTIVATION ARE INVOLVED IN EVENTS OF HEAT SHOCK- AND CARBONYL CYANIDE P-(TRIFLUOROMETHOXY) PHENYLHYDRAZONE (FCCP)- INDUCED APOPTOSIS ON BOVINE OOCYTES
Bibliographic record
Abstract
Activation of caspases is a highly recognized feature of apoptosis. Previously, we had showed that exposure of bovine oocytes to heat shock (HS) and carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) induced apoptosis and reduced subsequent embryonic development. Experiments were performed to: 1) determine whether apoptosis mediated by caspase-9 and/or caspase-3/7 are the causes for disruption of developmental competence of oocytes during maturation induced by HS and FCCP, to depolarize the mitochondria, and 2) whether inhibition of caspases in the oocyte prevents the reduced developmental potential caused by HS. Cumulus-oocyte complexes (COCs) were matured at 39°C (control) or 41°C (HS) as well as with 0.01% (v/v) DMSO (vehicle) or 10 μM FCCP at 39°C (FCCP) for 22h. All COC groups were fertilized and cultured in vitro at 39°C for 8 days. Exposure to FCCP or HS resulted in increased activity of caspase-9 and caspase-3/7 in oocytes. Nevertheless, at the blastocyst stage only caspase-3/7 activity was increased by both treatments. Further, COCs were placed in maturation medium with vehicle (0.01% [v/v] DMSO) or 200 nM of caspase inhibitors: z-LEHD-fmk, an inhibitor of caspase-9 activity; or z-DEVD-fmk, a caspase-3/7 inhibitor; or z-VAD-fmk, a broad caspase inhibitor. COCs were matured at 39°C or 41°C, fertilized and cultured at 39°C. In the presence of all inhibitors, HS oocyte development to the blastocyst stage was similar to control and showed reduced percentage of TUNEL-positive chromatin in oocytes and blastomeres. In conclusion, HS and FCCP induced oocyte mitochondrial dysfunction by activation of caspase-9 and caspase-3/7 also caspase inhibitors rescued the capacity of oocyte to support early embryonic development. (platform)
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".