DNA methylation and gene expression in the runt and methyl-deprived pig
Bibliographic record
Abstract
This research focuses on studying the effect of reduced maternal methyl donor supply as well as intrauterine growth restriction (IUGR) on DNA methylation and on gene expression in pigs. Two separate techniques were used to determine global DNA methylation levels (cytosine extension and methyl incorporation assays) but no differences at the genomic level were identified for either experimental group using either technique. The expression levels of numerous genes involved in methylation directly or whose expression might be expected to change under these experimental conditions were also investigated. The expression of DNMT3a2 and BHMT were significantly lower in B6-deprived pigs but no differences were observed in folate-deprived or runt pigs compared to relevant controls. Further, sex differences were observed, indicating that sex should be considered for future studies regarding methyl-donor or runt-large studies in pigs. Lastly, we discovered an aberrant RNA species (ARS) in numerous pigs in both of our studies, from both Newfoundland and Manitoba research pig populations, and this ARS significantly affected the expression of numerous genes in both control and treatment groups.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".