Estimating N<sub>2</sub>fixation in two species of<i>Alnus</i>in interior Alaska using acetylene reduction and<sup>15</sup>N<sub>2</sub>uptake
Bibliographic record
Abstract
:In interior Alaskan boreal forests two species of alder, Alnus tenuifolia and Alnus crispa, represent keystone species in floodplain and upland landscapes, respectively, due to the ability of these plants to form symbiotic associations with the nitrogen-fixing actinomycete, Frankia. It is believed that as much as 70% of the nitrogen (N) accumulated during the 200-y successional development of these forests is derived through atmospheric fixation by these species. Estimates of gross N inputs in these and many other ecosystems have traditionally utilized the acetylene reduction assay (ARA), which requires a conversion factor of the ratio of acetylene to N2 reduced by nitrogenase, the primary enzyme. Despite the fact that small variations in the reduction ratio can substantially influence estimates of N inputs, few studies have investigated how it varies spatially and temporally. The present study sought to 1) determine this conversion factor for both species of alder in situ by calibration of the ARA against a 15N2 uptake method we developed for field use and 2) determine whether the conversion factor varied with the successional stage in which the alders occurred. Averaged across all plants, the ratio of acetylene to N2 reduced was significantly greater in A. crispa. Significant differences in the value of the conversion factor were observed between early succession and later (mid and late) successional sites for both species. Such differences were also observed among replicate sites within and among stages. However, these stage and site differences may also be due to seasonal effects, which could not be controlled for with our design. Specific acetylene reductase activity (SARA) was only correlated with 15N2 uptake for early successional sites measured early in the growing season, when N2-fixation rates were lowest and the conversion factor was closest to the theoretical value of 4. A significant negative correlation was found between the conversion factor value and the rate of enzyme activity as determined by the 15N2 uptake method. Two hypotheses are proposed to explain this result: 1) that it is due to changes in the kinetic properties of nitrogenase at high levels of enzyme activity, resulting in an increased affinity of the enzyme for N2 relative to C2H2, and 2) that the concentration of C2H2 used in our ARA was insufficient to saturate nitrogenase.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".