GmCHS8 and GmIFS2 Gene Co-Determine Accumulation of Isoflavonoid in Soybean
Bibliographic record
Abstract
摘要： 大豆异黄酮受多基因控制，采用传统育种方法提高大豆异黄酮含量比较困难。我们之前的研究中表明，CHS8基因在异黄酮生物合成过程中发挥着重要作用，但CHS8基因过量表达并不能显著提高异黄酮含量。本研究利用Microarray技术，检测了高异黄酮品种RCAT Angra (RCAT)和低异黄酮品种Harovinton (HVNT)的18 362基因在种子发育过程中表达水平的变化以及大豆种子中异黄酮累积的趋势，利用RT-PCR证实CHS8和IFS2分别是CHSs和IFSs基因家族中的主要基因；证实CHS8是类苯基丙醇主路径中的主基因，并发现异黄酮支路中的IFS2基因在RCAT和HVNT品种中表达差异亦达到显著水平。进一步利用发根农杆菌转化系统，在大豆上分别过量表达CHS8、IFS2和CHS8+IFS2，前两者异黄酮含量较对照分别提高65.9%和34.4%，但增幅未达显著水平；而后者则提高了82.3%，增幅达显著水平(P<0.0001)，因而证实大豆中异黄酮的积累由CHS8和IFS2基因共同决定。
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".