Membrane Leakage and Antimicrobial Activity of Nylon-3 Polymers and Surfactants
Bibliographic record
Abstract
This study was part of an effort to gain insight into biological modes of action of membrane-active antimicrobial agents with vesicle leakage experiments. Specific systems tackled here were three nylon-3 type polymers, introduced by Prof. S.H.Gellman's group (U of Wisconsin at Madison), which have shown great potential to serve as antibacterials and fungicides. A comprehensive set of vesicle leakage experiments, performed on different lipid models, included (i) the fluorescence lifetime-based leakage assay, which yields not only the active concentration but also the mechanism of leakage (ii) kinetics of leakage, and (iii) concerted action tests with detergents. Results suggest that there are different, characteristic vesicle leakage patterns that can be assigned to specific modes of action. Vesicle data often, but not always, correlates with biological activity. We speculate that the correlation may depend on the type of leakage - some mechanisms of vesicle disruption may be more directly relevant in vivo than others.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.006 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".