1051 Effects of intrammamary LPS infusions on inflammation and reproductive parameters of dairy cows
Bibliographic record
Abstract
The objective was to determine the effects of LPS induced mastitis on systemic inflammatory response and early embryo development in lactating Holstein cows. Cows at 35 ± 7 DIM (n = 20) were submitted to a modified Double-Ovsynch program (10 d interval between protocols with CIDR and two PGF injections in the second protocol) and timed AI (d0). Cows were randomly assigned (block design) to two treatments: 1) LPS group- cows received an intrammamary infusions (d5 and d10) of 25 µg of LPS (strain 0111:B4) diluted in 10 mL of sterile saline at morning milkings; and 2) Control group- cows received infusion with saline. Blood samples were taken at different time intervals during the study to determine plasmatic concentrations of haptoglobin (Hp), tumoral necrosis factor α (TNF-α) and progesterone (P4). Milk samples were collected every 2 d for somatic cell count (SCC) and body temperature was recorded using a rumen-reticular bolus logger and summarized for every hour during the period. On Day 15 after AI, uterine flushing for embryo recover and interferon-tau (IFN-τ) measurement, as well as endometrium biopsy were performed. Data were analyzed using the MIXED procedure of SAS. Hp was greater in LPS compared with Control group (0.80 ± 0.06 vs. 0.45 ± 0.07; P < 0.01), but TNF-α concentration was similar (P = 0.72) between treatments. Milk production from d0 to d15 was greater for Control cows (37.5 ± 1.5 vs. 33.5 ± 1.3 kg/d; P < 0.01), whereas SCC was higher in LPS treated cows for about 48 h after each infusion (P < 0.01). Likewise, reticular temperature of LPS cows was elevated for 12 h (P < 0.01) after each infusion. Progesterone did not differ among treatments at all time collections (P = 0.72). However, pregnant cows had greater concentrations of progesterone on d 6, 9, and 10 post-AI (P < 0.01). The recovery rate was 55% and the length of recovered embryos (3.6 ± 0.9 vs. 2.4 ± 0.7 cm; P = 0.56) and IFN-τ concentration in the luminal uterine flushing (P = 0.44) were similar between treatments. In summary, the intrammamary infusion of LPS was able to trigger a systemic inflammatory response during post-AI period, but unable affect conceptus recovery and length, and intraluminal uterine IFN-t concentration.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".