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Record W2557458092 · doi:10.1107/s2053273314088391

First structure of a cGMP-dependent protein kinase

2014· article· en· W2557458092 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueActa Crystallographica Section A Foundations and Advances · 2014
Typearticle
Languageen
FieldChemistry
TopicSynthesis and Catalytic Reactions
Canadian institutionsStructural Genomics ConsortiumUniversity of Toronto
Fundersnot available
KeywordsAllosteric regulationCooperativityProtein kinase AcGMP-dependent protein kinaseMechanism (biology)KinasePlasmodium falciparumCell biologyProtein structureChemistryBiologyBiochemistryBiophysicsMitogen-activated protein kinase kinaseEnzymeMalariaImmunologyPhysics

Abstract

fetched live from OpenAlex

PKA and PKG are two of the eponymous members of the AGC family of protein kinases as well as two of the earliest protein kinases discovered. Due largely to contributions from structural biology, the mechanism of cooperative and allosteric regulation of PKA is well understood. On the other hand, no structure of PKG from any organism has been available until recently. We present the first ever structure of PKG from the malaria parasite Plasmodium falciparum. The structure features PKG in its auto-inhibited form - a pentagonal arrangement of 4 cGMP-binding sites and the catalytic kinase domain. By comparing of this structure with PKA structures, we propose a novel mechanism of cooperativity and allostery by which Plasmodium PKG is regulated.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.956
Threshold uncertainty score0.728

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.226
Teacher spread0.219 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it