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Record W2557605524

New Mutations: Condition-dependent Rates and Sex-specific Effects

2014· dissertation· en· W2557605524 on OpenAlexfundno aff
Nathaniel P. Sharp

Bibliographic record

VenueTSpace · 2014
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEpigenetics and DNA Methylation
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsGeneticsBiology
DOInot available

Abstract

fetched live from OpenAlex

Mutations arise spontaneously in all populations, and can severely diminish fitness. This mutation load is a key issue in evolutionary biology, affecting population persistence, the maintenance of genetic variation, and mating system evolution. The rate at which new mutations arise and their effects on fitness are generally treated as constant within a population, but theory suggests that variation in these parameters can have profound impacts. I explored the sex-specific effects of new mutations, and the role of condition in determining the mutation rate, using genotypes of Drosophila melanogaster loaded with spontaneous mutations. I found that the effect of mutations was more severe on male than on female fitness, pointing to a role for sexual selection in purging deleterious genetic variation. The expected consequence is that deleterious mutations will be eliminated at the expense of males, reducing the mutation load of sexual females, and thereby reducing the cost of sexual reproduction. I also found evidence that infection with a bacterial pathogen can heighten the degree of sex-specific selection. However, sex differences in selection could also reduce mean fitness if many alleles have sexually antagonistic effects. By comparing mutational and standing genetic variation, I tested for non-mutational variation in sexual fitness, which would be a signature of sexual antagonism. While there was some suggestion of sexual antagonism, deleterious mutations adequately explained levels of standing variation. Mutation load can also be affected if mutation rates depend on condition. I compared mutation rates across genotypes that varied in condition due to differences in genetic quality, and found that low condition caused elevated rates of mutational fitness decline. A genomic analysis revealed condition-dependent DNA repair pathway usage as the likely reason for this difference. Condition-dependent mutation is expected to reduce mean fitness in sexual populations, but may also increase the rate of extinction in asexuals.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.312
Teacher spread0.303 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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