The Survival of Malidixic Acid Resistance Genetic Marker of Escherichia Coli x1776 through the Gastrointestinal Tract of Mice
Bibliographic record
Abstract
Escherichia coli x17?6, a strain created from Escherichia coli K12, is used as an EK2 host (17) in plasmid engineering experiments. It is unable to survive passage through the intestinal tracts of rats at frequencies of less than 10-11 bacteria per milliliter because of several mutations that cause sensitivity to bile and requirements for diamino pimelic acid (DAP) and thymine (15). Although DNA degradation accompanies thymine-less death in in vitro experiments and is an all-or-none response for any given cell (15), it is likely that lysis associated with DAP-less death may liberate DNA prior to its degradation. Since undegraded recombinant DNA might be taken up by robust microorganisms encountered in the natural environment, especially in the intestinal tracts of humans after inadvertant ingestion by laboratory workers, by the mechanism of transformation, or as a consequence of concomitant phage infection, it is important to determine the "survival potential" of such DNA in non-laboratory controlled environments.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".