Effect of Bax G125A Single Nucleotide Polymorphism (SNP) on Bax Gene Expression in Diffuse Large Cell Lymphoma.
Bibliographic record
Abstract
Abstract Altered expression of Bax protein has been shown to be predictive of overall and disease free-survival of patients with diffuse large B-cell lymphoma (DLCL)(Am J Hematol, 2003; 73:101–7). Further, absence of Bax and low levels of Bcl-2 have been shown to be associated with a more aggressive phenotype (P<0.05) (Blood, 1997; 90:3173–8) in patients with DLCL. We have previously reported a single nucleotide polymorphim (SNP), G125A, in the 5′-untranslated region of the BAX promoter and its association with reduced protein expression and treatment resistance in B-cell chronic lymphocytic leukemia (Cancer Lett2002; 187:199–205). The aim of the present study was to evaluate BAX SNP incidence and its association with the protein expression in lymphomas. DNA was extracted from fifty lymphoma samples (31 DLCL, seven follicular lymphomas (FL), three marginal zone lymphomas (MZL), four T-cell lymphomas, three Hodgkin’s lymphomas, and two cases of Mantle cell lymphoma (MCL), and from 55 control samples. BAX SNP was detected by restriction enzyme analysis (J Clin Pathol, 2003; 56: 205–9). Fourteen out of 50 (28%) samples had G125A SNP, in all cases only one allele was replaced. It was present in eight (26%) DLCL, two (29%) FL, in two MZL, in one T-cell lymphoma, in one HL, and none of MCL had this SNP. Eight out of 55 (14.5%) controls had the SNP. The presence of BAX promoter SNP was associated with reduced Bax protein expression as detected by Western blot analysis (P=0.024). Two plasmids with G and A nucleotides at position 125 of the BAX promoter linked to luciferase as a reporter were constructed and used for transfection, as well as the pGL3-Basic and pGL3-Control plasmids; pRL-SV40 plasmid was used for co-transfection. Dual-Glo luciferase assay was performed and Firefly luciferase activity was normalized to Renilla luciferase. In transfection experiments, the G125A SNP reduced expression (P=0.0202) of BAX promoter. BAX SNP, G125A, present in approximately a quarter of DLCL has a direct negative effect on BAX gene expression.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".