Preparation of Nano-Sized Mesoporous Silica and Its Application in Immobilization of β-galactosidase from Aspergillus Oryzae
Bibliographic record
Abstract
In alkaline conditions, monodisperse nano-sized mesoporous silica was synthesized using cetyl trimethyl ammonium bromide (CTAB) as template and tetraethoxysilane (TEOS) silica as source in ethanol / water cosolvent conditions. Using method of nitrogen adsorption, specific surface area of the dried monodisperse nano-sized mesoporous silica was about 1591 m2/g and the pore size was about 3.8 nm. The field-emission scanning electron microscope (SEM) micrographs showed that the silica particles obtained were spherical with an approximate diameter of 160 nm and of good dispersion. Transmission electron microscopy (TEM) revealed that the carrier had an excellent cellular structure with disordered multi-channels and smooth surface. The nano-sized mesoporous silica above was employed to immobilize β-galactosidase from aspergillus oryzae for the first time. At the experimental conditions in section 2.4, the enzyme activity and the activity yield were 535.11 U/g dry carrier and 79.63%, respectively. Kinetic data of the immobilized enzyme such as optimum temperature, pH, and thermal and pH stability among other valuable results were also determined.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".