ISDN2014_0068: Identification of a novel population of V2 interneurons in the developing mouse spinal cord
Bibliographic record
Abstract
Locomotion is regulated in the spinal cord by Central Pattern Generators (CPGs) that control rhythmicity, left/right and flexor/extensor alternation, regularity and speed of the cycles of limb muscle contractions. These circuits are constituted by spinal motor neurons and by several interneuron populations generated during embryonic development in the ventral and in the dorsal portions of the developing spinal cord. Ventral interneurons derive from 4 cardinal populations designated V0 to V3, which diversify into multiple subsets characterized by specific molecular identity, migration pathway, final location, axonal targeting, neurotransmitters and activity in the CPGs. V2 interneurons comprise V2a and V2b cells generated in equal amount through a Notch-dependant mechanism, and V2c interneurons that seem related to V2b cells. Here, we report that an additional early subset of V2 interneurons is generated in the developing mouse spinal cord. These cells are produced from embryonic day 9.5 onward from the p2 progenitor domain, i.e. at a stage when the other V2 subsets are not detectable yet. They display a molecular identity distinct from the other ventral interneuron populations. Until e11.5, they are tightly intermingled with and as numerous as the V2a and V2b cells. However, analyzing several mouse mutant lines, we demonstrate that the generation of these cells does not depend on Notch signalling nor on other known developmental determinants of V2 interneurons, including Ascl1 and Foxn4. Hence, we demonstrate the existence of a subset of V2 interneurons generated early during neuronal differentiation in the spinal cord. However, lack of a permanent marker for these cells currently prevents any identification of its progeny or of the role exerted by these cells in the regulation of the locomotion.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".