Abstract P250: Central Mechanisms Mediating Angiotensin II-Salt Hypertension in Rats
Bibliographic record
Abstract
Circulating Ang II causes persistent activation of brain angiotensinergic pathways through a neuromodulatory aldosterone (aldo)-mineralocorticoid receptors (MR)-epithelial Na + channel (ENaC)-ouabain pathway. The response of BP to circulating Ang II is enhanced by high salt intake. To evaluate the central mechanisms that mediate Ang II-salt induced hypertension, Wistar rats were treated with regular salt diet (0.4% NaCl), high salt diet (2% NaCl), sc Ang II (150 ng/kg / min), or sc Ang II with high salt diet for 14 days. In the 2 nd exp, MR blockers (eplerenone, spironolactone), ENaC blocker (benzamil), AT 1 R blocker (losartan) or vehicles (Veh) were icv infused combined with Ang II-salt. BP was recorded by telemetry. Gene expression was assessed by real-time qPCR. Plasma Ang II and tissue aldosterone were measured by RIA. Ang II alone caused a small increase in MAP (112±1 vs 99±1 mmHg), and BP was markedly increased by Ang II-salt (152±4 mmHg, P<0.05 vs. others). BP increases to Ang II-salt were largely inhibited by central infusion of MR blockers (103±2mmHg), benzamil (100±3mmHg) or losartan (98±4mmHg). Ang II alone or together with salt decreased 11βHSD2 and MR mRNA expression but increased AT 1 R and ENaC γ mRNA expression in the PVN, and increased AT 1 R mRNA level in the RVLM. Sc Ang II or high salt diet had no effect on mRNA levels of CYP11B1, B2, ENaC α or β in the PVN or RVLM. Considering that AT 1 R mRNA expression increases in both PVN and RVLM and central MR-ENaC-AT 1 R blockade prevents the hypertension, these results suggest that activation of Ang II-AT 1 R signaling and MR-ENaC pathway in the brain contribute to both Ang II and Ang II-salt hypertension.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.003 |
| Insufficient payload (model declined to judge) | 0.006 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".