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Record W2582518578 · doi:10.1073/pnas.1613609114

Single polysaccharide assembly protein that integrates polymerization, termination, and chain-length quality control

2017· article· en· W2582518578 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEnzyme Production and Characterization
Canadian institutionsAlberta Glycomics CentreUniversity of AlbertaUniversity of Guelph
FundersNatural Sciences and Engineering Research Council of CanadaGovernment of Canada
KeywordsGlycanBiochemistryGlycosyltransferaseGlycoconjugatePolymeraseATP-binding cassette transporterOperonPolysaccharideBacterial outer membraneBiosynthesisBiologyTransferaseChemistryTransporterEnzymeGeneGlycoproteinEscherichia coli

Abstract

fetched live from OpenAlex

Significance Glycosyltransferase enzymes synthesize complex sugar-containing macromolecules that play pivotal roles in the biology of all cells. Bacteria produce a vast range of glycoconjugate structures that frequently dictate host–pathogen interactions. The composition and chain length of bacterial glycans are both important for biological function, yet the mechanisms for chain-length determination are generally poorly understood. Here, we describe a remarkable family of proteins in which each member contains multiple glycosyltransferase modules (often representing new glycosyltransferase families) for glycan polymerization and chain termination. The polymerase and terminator activities are separated by a molecular ruler that establishes product length. The modularity of these proteins provides an adaptable platform for generating antigenic diversity in nature and potential new strategies for glycoengineering.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.139
Threshold uncertainty score0.275

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.038
GPT teacher head0.311
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it