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Record W2586290791 · doi:10.1152/ajpcell.00219.2015

Identification of a mammalian silicon transporter

2017· article· en· W2586290791 on OpenAlex
Sarah J. Ratcliffe, Ravin Jugdaohsingh, Julien Vivancos, Alan O. Marron, Rupesh Deshmukh, Jian Feng, Namiki Mitani‐Ueno, Jack Robertson, John W. Wills, Mark V. Boekschoten, Michael Müller, Robert C. Mawhinney, Stephen D. Kinrade, Paul Isenring, Richard R. Bélanger, Jonathan J. Powell

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueAmerican Journal of Physiology-Cell Physiology · 2017
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAluminum toxicity and tolerance in plants and animals
Canadian institutionsHôtel-Dieu de QuébecLakehead UniversityHealth CanadaUniversité Laval
FundersDivision of Mathematical SciencesFaculty of Medicine and Health, University of SydneyNatural Sciences and Engineering Research Council of CanadaDirectorate for Biological SciencesFitzwilliam College, University of CambridgeCanadian Institutes of Health ResearchMedical Research CouncilUniversity College LondonMinistry of Education, Culture, Sports, Science and TechnologyBiotechnology and Biological Sciences Research CouncilGovernment of CanadaUniversity of BristolUniversity of East Anglia
KeywordsTransporterDownregulation and upregulationEffluxCell biologyExtracellularCotransporterBiologyTransport proteinBiochemistrySymporterHomeostasisXenopusPhosphateChemistrySodiumGene

Abstract

fetched live from OpenAlex

Silicon (Si) has long been known to play a major physiological and structural role in certain organisms, including diatoms, sponges, and many higher plants, leading to the recent identification of multiple proteins responsible for Si transport in a range of algal and plant species. In mammals, despite several convincing studies suggesting that silicon is an important factor in bone development and connective tissue health, there is a critical lack of understanding about the biochemical pathways that enable Si homeostasis. Here we report the identification of a mammalian efflux Si transporter, namely Slc34a2 (also termed NaPiIIb), a known sodium-phosphate cotransporter, which was upregulated in rat kidney following chronic dietary Si deprivation. Normal rat renal epithelium demonstrated punctate expression of Slc34a2, and when the protein was heterologously expressed in Xenopus laevis oocytes, Si efflux activity (i.e., movement of Si out of cells) was induced and was quantitatively similar to that induced by the known plant Si transporter OsLsi2 in the same expression system. Interestingly, Si efflux appeared saturable over time, but it did not vary as a function of extracellular [Formula: see text] or Na + concentration, suggesting that Slc34a2 harbors a functionally independent transport site for Si operating in the reverse direction to the site for phosphate. Indeed, in rats with dietary Si depletion-induced upregulation of transporter expression, there was increased urinary phosphate excretion. This is the first evidence of an active Si transport protein in mammals and points towards an important role for Si in vertebrates and explains interactions between dietary phosphate and silicon.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.770
Threshold uncertainty score0.300

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.232
Teacher spread0.219 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it