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Record W2588549316 · doi:10.1109/tps.2017.2657748

External AC Electric Field-Induced Conformational Change in Bovine Serum Albumin

2017· article· en· W2588549316 on OpenAlexfundno aff
Takamasa Okumura, Kazuki Yamada, Taro Yaegashi, Katsuyuki Takahashi, Bunei Syuto, Koichi Takaki

Bibliographic record

VenueIEEE Transactions on Plasma Science · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial Inactivation Methods
Canadian institutionsnot available
FundersJapan Society for the Promotion of ScienceCanadian Association for the Study of the Liver
KeywordsBovine serum albuminTrypsinizationSodium dodecyl sulfateTrypsinConformational changeChemistryGel electrophoresisElectric fieldBiophysicsElectrophoresisChromatographyBiochemistryBiologyEnzyme

Abstract

fetched live from OpenAlex

An ac electric field (acEF) application technology has been reported as a principle technique for food preservation. With this method, conformational changes in the protein molecules of food materials may be induced. Conformational changes were experimentally examined using bovine serum albumin (BSA) as a model protein using an acEF technology. The amplitude of acEF was 66.7 kV/m. BSA with acEF (ac-BSA) and BSA without the ac field (n-BSA) were trypsinized under the same conditions. The derived peptides were compared by using sodium dodecyl sulfate polyacrylamide gel electrophoresis patterns. Formation of tryptic peptides was increased in n-BSA, and trypsin digestion was suppressed in ac-BSA. These results indicate that conformational changes in BSA molecules were induced by acEF application.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.337
Teacher spread0.297 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations16
Published2017
Admission routes1
Has abstractyes

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