AN EFFICIENT METHOD FOR CRYOPRESERVATION OF ST JOHN¿S WORT AND TOBACCO: ROLE OF MELATONIN
Why this work is in the frame
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Bibliographic record
Abstract
International trade in medicinal plants is a major force in the current global economy and the demand of natural health products is increasing in developing and industrialized nations. Unfortunately, one third of all plant species and medicinal plants in particular, are under threat of extinction due to indiscriminate harvest, diseases, and reduced habitats. In vitro culture and cryopreservation techniques can ensure safety of these species and have potential applications in sustainable production and use of plant based medicines. The objective of this study was to develop an efficient cryopreservation protocol for in vitro maintained lines of medicinal plants using St John's Wort and tobacco as experimental systems. Shoot tips of in vitro-grown plantlets were successfully cryopreserved in liquid nitrogen at -196°C under controlled environment conditions following melatonin treatment and cold acclimation (CA) with either vitrification or encapsulation-vitrification methods. The explants showed optimal re-growth following cryopreservation when treated with the Plant Vitrification Solution #2 (PVS2) for 20 min on ice. Addition of the antioxidant melatonin (0.1-0.5 µM) added to both pre-culture and re-growth media significantly improved the recovery of plants with 80 to 100% re-growth of cryopreserved shoots. The melatonin based protocol for improved recovery of cryopreserved tissue may serve as an important tool for long-term storage and security of germplasm of medicinal plant species.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it