Anti-Mullerian Hormone Inhibits FSH-Induced Adenylyl Cyclase Activation, Aromatase Expression, and Estradiol Production in Human Granulosa-Lutein Cells.
Bibliographic record
Abstract
Anti-Mullerian hormone (AMH) is a member of the transforming growth factor-β superfamily of growth and differentiation factors. Although it is clear that AMH attenuates the effects of follicle-stimulating hormone (FSH) on follicle development, little is known about the function that AMH exerts on steroidogenesis in female human gonadal cells. Thus, the aims of this study were to investigate the effects of AMH on basal and FSH-induced aromatase activity and estradiol production in human granulosa-lutein cells, and to elucidate the mechanism by which AMH exerts its effects. Blood samples and human granulosa-lutein cells were obtained from consenting in vitro fertilization patients in an academic center. Analysis of patient serum estradiol and AMH levels by enzyme immunoassay revealed a negative correlation between the two on the date of human chorionic gonadotropin administration. Next, we used the human granulosa-lutein cell model to examine the effects of treatment with AMH (10 ng/mL) on basal and FSH-stimulated (0.2 IU/mL) estradiol accumulation as well as aromatase mRNA and protein levels. Treatment with AMH significantly reduced FSH-stimulated aromatase mRNA and protein levels; whereas it had no measurable effects on basal or 8-Br-cyclic adenosine monophosphate-stimulated levels. Similarly, treatment of human granulosa-lutein cells with AMH significantly reduced FSH-stimulated estradiol accumulation, but did not affect basal estradiol production. FSH receptor mRNA levels were not altered in AMH-treated cells suggesting effects at the level of adenylyl cyclase. Indeed, pre-treatment of human granulosa-lutein cells with AMH suppressed FSH-induced increases in intracellular cyclic adenosine monophosphate levels. These results suggest that AMH inhibits FSH-induced aromatase expression and estradiol production in human granulosa cells by reducing adenylyl cyclase activation. This study was supported by the Canadian Institutes of Health Research. (poster)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".