Bibliographic record
Abstract
Characterization of virulence factors in P. falciparum malaria is essential in order to identify new therapeutic and prophylactic targets. Rosette formation, the binding of uninfected red blood cells to parasite-infected red blood cells, is a P. falciparum virulence phenotype associated with severe clinical manifestations, e. g. cerebral malaria and severe anemia. Humoral responses to rosetting epitopes seem to confer protective immunity and we have shown in these studies that individuals living in malaria endemic areas build up age-related humoral responses to rosetting epitopes that parallel the development of clinical immunity. Furthermore, epitopes that mediate the rosetting phenotype appear to be diverse but conserved between distant regions in Africa. We have performed a detailed study of the cell-cell binding mechanisms that govern rosetting. Polysaccharides belonging to the glycosaminoglycan family, especially heparin and heparan sulfate, inhibit rosette formation and treatments of host cells indicate that heparan sulfate-like glycans support adhesion. In addition, the ABO blood group phenotype of the infected host modulates rosetting and blood group A and B antigens have been shown to function as co-receptors to other receptors in rosetting. The parasite-derived adhesion molecule that mediates rosetting has been identified as a Plasmodium falciparum erythrocyte membrane protein I (PfEMP1) variant, a product of the vast family of var genes. PfEMP1 mediates adhesion by interacting with host cell glycan receptors, such as heparan sulfate and blood group A antigen. We have characterized the binding of heparan sulfate and heparin to the rosetting domain of PfEMP1. Important molecular features of oligosaccharides required for optimal binding, such as molecular size (12-mer oligosaccharide chain or larger) and N-sulfation, have thus been identified. In field studies, the heparin-binding phenotype of P. falciparum was found to be more common among patients with severe malaria. Furthermore, the clinical isolates had the ability to adhere to multiple receptors. We have characterized the binding properties of PfEMP1. to multiple host receptors, which may explain the poly-adhesive P. falciparum phenotype associated with severe disease in these studies. The present investigation contributes to the molecular elucidation of virulent adhesive phenotypes in P. falciparum malaria. The identified molecules involved in the cell-cell adherence may serve as targets for prophylactic and therapeutic measures. List of scientific papers I. Barragan A, Kremsner PG, Weiss W, Wahlgren M, Carlson J (1998). Age-related buildup of humoral immunity against epitopes for rosette formation and agglutination in African areas of malaria endemicity. Infect Immun. 66(10):4783-4787. https://pubmed.ncbi.nlm.nih.gov/9746579 II. Chen Q, Barragan A, Fernandez V, Sundström A, Schlichtherle M, Sahlén A, Carlson J, Datta S, Wahlgren M (1998). Identification of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) as the rosetting ligand of the malaria parasite P. falciparum. J Exp Med. 187(1):15-23. https://pubmed.ncbi.nlm.nih.gov/9419207 III. Barragan A, Spillmann D, Kremsner PG, Wahlgren M, Carlson J (1999). Plasmodium falciparum: molecular background to strain-specific rosette disruption by glycosaminoglycans and sulfated glycoconjugates. Exp Parasitol. 91(2):133-143. https://pubmed.ncbi.nlm.nih.gov/9990341 IV. Barragan A, Fernandez V, Chen Q, von Euler A, Wahlgren M, Spillman D. The duffy-binding-like domain 1 of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a heparan sulfate ligand that requires 12-mers for binding. [Submitted] V. Heddini A, Obiero J, Barragan A, Kai O, Wahlgren M, Marsh K. Plasmodium falciparum-infected erythrocytes of children with servere malaria bind to multiple receptors. [Submitted] VI. Chen Q, Heddini A, Barragan A, Fernandez V, Pearce SFA, Wahlgren M. The semi-conserved head structure of Plasmodium falciparum erythrocyte membrane protein 1 mediates binding to multiple independent host receptors. [Submitted] VII. Barragan A, Kremsner P, Wahlgren M, Carlson J (2000). Blood group A antigen is a co-receptor in Plasmodium falciparum rosetting. Infection and Immunity. [Accepted] https://pubmed.ncbi.nlm.nih.gov/10768996
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".