Oxytocin and Interferon-Tau Regulation of Prostaglandin Transporter (PGT) and Multidrug Resistant Associated Protein 4 (MRP4) in the Bovine Endometrium.
Bibliographic record
Abstract
Prostaglandins (PG) are involved in many female reproductive processes and are regulated at the levels of biosynthesis, secretion and catabolism. Transmembrane transport of PGs is also an important site of regulation and we have shown the requirement of PG transporter (PGT/SLCO21) for functional luteolysis in the bovine. A comprehensive screening of the endometrial expression of different PGTs indicated that MRP4/ABCC4 of ATP Binding Cassette (ABC) family was regulated during the bovine estrous cycle apart from the previously studied PGT/SLCO21 of Solute Carrier Organic Anion family. Accordingly, we have confirmed the expression of these carriers in our recently developed endometrial epithelial and stromal cell lines bEEL and CSC. Using these models, we have studied the effect of oxytocin (OT) and/or interferon tau (IFNt) and phorbol myristoyl acetate (PMA) on the regulation of PGT and MRP4 in relation with PGs production. Downregulation of MRP4 expression using siRNA induced a significant increase of PGF2α accumulation relative to PGE2 in the culture medium of CSC. Treatment of bEEL cells with OT stimulated COX2 expression and PGF2αproduction. These responses correlated well with upregulation of MRP4/ABCC4 but not PGT/SLCO2A1. In contrast, IFNt at a concentration that stimulated COX-2 without a corresponding increase in PG accumulation stimulated the expression of PGT/SLCO2A1 but not that of MRP4/ABCC4. Inhibition of OT induced PG production by the COX2 inhibitior NS-398 did not affect the induction of ABCC2 or COX2 expression in bEEL cells. Since OT at the origin of luteolytic PGF2α regulates MRP4 and IFNt, the embryonic antiluteolytic signal, regulates PGT, it is possible that these modulators are involved in selective or preferential transport of terminal PGs like PGE2 and PGF2α. (poster)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".