Sertoli and Granulosa Cell-Specific Vascular Endothelial Growth Factor (VEGF) Loss Using pDMRT-1 Cre Alters Gonadal Morphogenesis and Function.
Bibliographic record
Abstract
Inhibition of VEGF disrupts both testis-specific vascular development and cord formation. In the female, VEGF isoforms regulate follicle development and may affect the initial primordial follicle pool. Therefore, the objective of the current study was to evaluate effects of Sertoli and Granulosa cell-specific production of VEGF isoforms on vascular development and gonadal morphogenesis. The pDmrt1 gene is expressed in the indifferent gonad at 10.5 dpc in precursor Sertoli and Granulosa cells. Therefore, we used a DMRT1-cre crossed to a floxed VEGF mouse to determine potential phenotypes in male Sertoli-cell (VEGF-DSert) and female Granulosa-cell (VEGF-DGran) specific knockouts. Mice from these crosses had litters and had approximately one less mouse/litter than controls. Reproductive organs were collected from adult mice, and weighed (male: control n=9, VEGF-DSert; n=8; female: control n=11, VEGF-DGran n=10). Testosterone and estrogen levels in blood were determined by ELISA. In VEGF-DSert , the weight of the body (28.52±0.48 VS 32.52±1.20 g); testes (0.1658±0.0064 VS 0.1924±0.0061 g) and epididymus (0.0627±0.0046 VS 0.0792±0.0042 g) were significantly smaller than those from the control (P<0.05). The prostate weight also tended to be different (P=0.0636) than controls. Testosterone was not different when averaged across treatment and controls groups; however, three VEGF-DSert males had 10 fold greater testosterone concentrations than controls (12.81 vs 1.18 ng/ml). For the females, the weight of ovaries from VEGF-DGran were significantly smaller than that of the control (0.0144±0.0014 VS 0.0183±0.0009 g, P<0.05). Two VEGF-DGran ovaries had abnormal morphology in structures that appeared to be CL. Furthermore, the estrogen concentrations in the VEGF-DGran mice were 56% lower than those in control mice (48.83±9.58 VS 91.53±14.75 pg/ml, P<0.05). Therefore, we can conclude from these data that lack of VEGF in Sertoli and Granulosa cells alters reproductive organ morphogenesis, reduces gonadal and male accessory gland organ weights, alters steroidogenic profiles and potentially causes subfertility in both male and female mice. This research was supported by NIH/NICHD HD051979. (poster)
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".