120 After all, tomorrow is another day for the transition cow: Depending on liver and reproductive health, of course
Bibliographic record
Abstract
Infertility in lactating dairy cows has been attributed to metabolic stress during the transition period. Potential metabolic alterations that dysregulate ovarian functions have not been completely cataloged. Our objective was to characterize metabolic parameters of dairy cows during the transition period. First, we examined the metabolic profiles in circulation to pinpoint time points of major changes. We collected weekly blood samples from Holstein cows (N = 15) from 3 wk before to 12 wk after calving. Glucose levels reduced during pre-calving weeks to reach a nadir at 3 wk post-calving (P < 0.05), and the first increase in glucose concentration occurred at 10 wk post-calving (P < 0.05). Also, β-hydroxybutyric acid levels increased from calving until week 3 of lactation (P < 0.05) and subsequently returned to baseline. Levels of triglycerides decreased during pre-calving weeks, while significant increase occurred at 5 wk post-calving (P < 0.05). Total cholesterol concentrations increased from the third to seventh week post-calving (P < 0.05). Oxidative stress indicator, glutathione, decreased to reach a nadir by 7 wk in lactation (P < 0.05). Thus, post-calving weeks 3 to 7 are associated with major alterations in metabolic indicators in circulation. In the second experiment, we evaluated changes in hepatic and granulosa cell (GC) mRNA levels and circulating metabolic indicators during the periods of major metabolic changes listed above. We collected blood and liver biopsies from Holstein cows at 3 wk pre-partum, during calving week, and 7 wk post-partum; this last liver sample accompanied collection of GCs from the dominant follicle by follicular aspiration. Cows were separated into two groups, where Group 1 (N = 4) consisted of cows that lost less than 0.75 body condition score (BCS) during the sampling trial and Group 2 (N = 4) consisted of cows that lost equal to or greater than 0.75 BCS. Lipid metabolism and oxidative stress were evaluated in hepatic tissue by qPCR. Transcript abundance revealed a decrease in CYP7A1 (P < 0.05) and tendency for decrease of LDLR (P < 0.1) in cows from Group 2 at 3 wk pre-partum. Transcript abundance of SRB1, ACAT1, PON1, and APOB100 remained unaltered between groups at all time points (P > 0.05). Assessing reproductive competence through mRNA abundance of genes required for growth and ovulation (SRB1, FSHR, and LHR) in GCs remained unchanged (P > 0.05). Taken together, transition period features increasing cholesterol, triglycerides and decreasing glucose and glutathione compared to pre-calving period. Further studies, including liver function assays, are required to thoroughly investigate the relationship between liver health, reproductive health, and alterations in circulating metabolic indicators.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".