Mitochondria Modulates Calcium Pulsars In Native Endothelial Cells
Bibliographic record
Abstract
Calcium (Ca 2+ ) homeostasis is critical to endothelial function. Indeed, localized endothelial Ca 2+ signals, such as Ca 2+ pulsars ‐ restricted IP 3 R‐dependent Ca 2+ releases within myoendothelial projection (MEP) ‐ emerge as major modulators of vascular tone. However, Ca 2+ pulsars regulation remains yet unexplored. Conversely, mitochondria appear to be an important regulator of intracellular Ca 2+ signals in several cell types. We thus hypothesized that mitochondria regulates endothelial Ca 2+ pulsar. Electron microscopy first showed an asymmetrical mitochondrial distribution in endothelium of mice mesenteric arteries, with a higher mitochondrial density near MEP. High‐speed confocal microscopy was next used to study the impact of mitochondria on Ca 2+ pulsars. Mitochondrial uncoupling by FCCP application unveiled pulsars active sites (+60%) but decreased discrete site frequency (‐30%). Moreover, pharmacological alteration of mitochondrial Ca 2+ import/export mechanisms (Kaempferol, Ru360, CGP37157) had a significant impact on Ca 2+ pulsar dynamics. In summary, near‐MEP mitochondria might be an important modulator of Ca 2+ pulsars and hence of endothelial function. Support: FRQS, HSFC, CFI, SQHA and CIHR.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".