Expression and Characterization of Serotype 2 <b><i>Streptococcus suis </i></b>Arginine Deiminase
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
<b><i>Background:</i></b> Arginine deiminase (ArcA) has been speculated to facilitate the intracellular survival of <i>Streptococcus suis</i> under acidic conditions. However, the physical and biological properties and function of <i>SS2</i>-ArcA have not yet been elucidated. <b><i>Methods:</i></b> Recombinant <i>SS2</i>-ArcA (r<i>SS2</i>-ArcA) was expressed and purified using Ni-NTA affinity chromatography. Under various pH and temperature conditions, the enzymatic properties of purified r<i>SS2</i>-ArcA and crude native <i>SS2</i>-ArcA were determined. <b><i>Results:</i></b> The <i>SS2</i>-<i>arcA</i>-deduced amino acid sequence contained a conserved catalytic triad (Cys399-His273-Glu218). The optimum temperature and pH of 47-kDa r<i>SS2</i>-ArcA and crude native <i>SS2-</i>ArcA were 42°C and pH 7.2. The r<i>SS2</i>-ArcA and crude native <i>SS2</i>-ArcA were stable for 3 h at 4 and 25°C, respectively. The pH stability and dependency tests suggested that r<i>SS2</i>-ArcA and crude native <i>SS2</i>-ArcA were functionally active in acidic conditions. The <smlcap>L</smlcap>-arginine substrate binding affinity (<i>K</i><sub><i>m</i></sub>) values of r<i>SS2</i>-ArcA (specific activity 16.00 U/mg) and crude native <i>SS2-</i>ArcA (specific activity 0.23 U/mg) were 0.058 and 0.157 m<smlcap>M</smlcap>, respectively. r<i>SS2</i>-ArcA exhibited a weak binding affinity with the common ArcA inhibitors <smlcap>L</smlcap>-canavanine and <smlcap>L</smlcap>-NIO. Furthermore, the partial inactivation of <i>SS2</i>-ArcA significantly impaired the viability and growth of <i>SS2</i> at pH 4.0, 6.0, and 7.5. <b><i>Conclusions:</i></b> This study profoundly demonstrated the involvement of ArcA enzymatic activity in <i>S. suis</i> survival under acidic conditions.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it