463 Broiler chickens express differential alkaline phosphatase activity and enzyme affinity in hydrolyzing ATP along the small intestinal longitudinal axis
Bibliographic record
Abstract
Broiler chicken growth rates are rapidly accelerating in the industry, requiring an average of 6 to 7 weeks to achieve market body weight. A possible determinant is intestinal alkaline phosphatase (IAP), a key enzyme which dephosphorylates pathogen-associated-molecular patterns (PAMPs) such as endotoxin lipopolysaccharides (LPS) and ATP, thereby preventing gut dysbosis and disorders. However, IAP's roles in maintaining gut health have yet to be elucidated in the broiler chicken. Thus, the objective of this study was to determine IAP activity kinetics for hydrolyzing ATP at pH 7.4 and 41°C in the duodenal, jejunal, and ileal segments of young broilers. Ten 28-d old broiler (Ross 708) chickens, maintained on a standard grower corn soybean meal diet, were euthanized for collecting duodenal, jejunal, and ileal tissue samples. Each ATP kinetic experiment was conducted with 20 gradient concentrations of ATP in 4 replicates, ranging 0 – 5 mM in incubation media. All enzymatic incubations were conducted with about 3 µg protein of the homogenized small intestinal segmental tissues. The kinetics (parameter estimates ± SE, P < 0.05, R2 = 0.82 – 0.93; n = 80) of the broiler small intestinal segmental IAP activities of hydrolyzing ATP were obtained, including Vmax values (µg/mg protein·min) of 7.59 ± 0.09, 8.60 ± 0.16, and 7.77 ± 0.19; and Km values (mM) of 0.045 ± 0.004, 0.132 ± 0.015, and 0.105 ± 0.015 in the duodenum, jejunum, and ileum, respectively. Pooled t-tests showed that the Vmax was higher (P < 0.05) in the jejunum than in the duodenum and the ileum, while the Vmax value was higher (P < 0.05) in the ileum than in the duodenum. Furthermore, the Km value was lower (P < 0.05) in the duodenum than in the jejunum by 2-fold and the ileum by 1.3-fold, respectively, while the Km value was lower (P < 0.05) in the ileum than in the jejunum by 26%. Our results suggest that young broiler chickens express differential alkaline phosphatase activity and enzyme affinity in hydrolyzing ATP along the small intestinal longitudinal axis.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".