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Record W2608545378 · doi:10.1038/ncomms15034

Functional characterization of a multi-cancer risk locus on chr5p15.33 reveals regulation of TERT by ZNF148

2017· article· en· W2608545378 on OpenAlexaff
Jun Fang, Jinping Jia, Matthew Makowski, Mai Xu, Zhaoming Wang, Tongwu Zhang, Jason W. Hoskins, Jiyeon Choi, Younghun Han, Mingfeng Zhang, Janelle Thomas, Michael A. Kovacs, Irene Collins, Marta Dzyadyk, Abbey Thompson, Maura O’Neill, Sudipto Das, Qi Lan, Roelof Koster, Federico Canzian, Charles Kooperberg, Alan A. Arslan, Paige M. Bracci, Julie E. Buring, Eric J. Duell, Steven Gallinger, Eric J. Jacobs, Aruna Kamineni, Stephen Van Den Eeden, Alison P. Klein, Laurence N. Kolonel, Donghui Li, Sara H. Olson, Harvey A. Risch, Howard D. Sesso, Kala Visvanathan, Wei Zheng, Demetrius Albanes, Melissa A. Austin, Marie‐Christine Boutron‐Ruault, H. Bas Bueno‐de‐Mesquita, Michelle Cotterchio, J. Michael Gaziano, Edward L. Giovannucci, Michael Goggins, Myron D. Gross, Manal M. Hassan, Kathy J. Helzlsouer, Elizabeth A. Holly, David J. Hunter, Mazda Jenab, Rudolf Kaaks, Timothy J. Key, Kay‐Tee Khaw, Vittorio Krogh, Robert C. Kurtz, Andrea Z. LaCroix, Loı̈c Le Marchand, Satu Männistö, Alpa V. Patel, Petra H. Peeters, Elio Ríboli, Xiao-Ou Shu, Malin Sund, Mark Thornquist, Anne Tjønneland, Geoffrey S. Tobias, Dimitrios Trichopoulos, Jean Wactawski‐Wende, Herbert Yu, Kai Yu, Anne Zeleniuch‐Jacquotte, Robert N. Hoover, Patricia Hartge, Charles S. Fuchs, Stephen J. Chanock, Victoria L. Stevens, Neil E. Caporaso, Paul Brennan, James McKay, Xifeng Wu, Heike Bickeböller, Angela Risch, Erich Wichmann, Richard S. Houlston, Graham J. Mann, John L. Hopper, Joanne F. Aitken, Bruce K. Armstrong, Graham G. Giles, Elizabeth A. Holland, Richard Kefford, Anne Ε. Cust, Mark A. Jenkins, Helen Schmid, Susana Puig, Paula Aguilera, Célia Bádenas, Alicia Barreiro, Cristina Carrera, Daniel Gabriel, Pol Giménez‐Xavier, Pablo Iglesias-García, Josep Malvehy, Montserrat Milà, Ramón Pigem, Míriam Potrony, Joan Anton Puig-Batille, Gemma Tell‐Martí, Nicholas K. Hayward, Nicholas G. Martin, Grant W. Montgomery, David L. Duffy, David C. Whiteman, Stuart MacGregor, Donato Calista, Giorgi Landi, Paola Minghetti, Fabio Arcangeli, Pier Alberto Bertazzi, Paola Ghiorzo, Giovanna Bianchi‐Scarrà, Lorenza Pastorino, William Bruno, Virginia Andreotti, Paola Queirolo, Francesco Spagnolo, Rona M. MacKie, Julie Lang, Nelleke A. Gruis, Frans A. van Nieuwpoort, Coby Out, Wilma Bergman, Nicole A. Kukutsch, Jan Nico Bouwes Bavinck, Egbert Bakker, Nienke van der Stoep, Jeanet ter Huurne, Han van der Rhee, Marcel W. Bekkenk, Dyon Snels, Marinus van Praag, Liève Brochez, Rianne M. J. P. Gerritsen, Marianne B. Crijns, Hans F. A. Vasen, Bart Janssen, Christian Ingvar, Håkan Olsson, Göran Jönsson, Åke Borg, Katja Harbst, K. Nielsen, Anita Schmidt Zander, Anders Molvern, Per Helsing, Per Arne Andresen, Helge Rootwelt, Lars A. Akslen, Brigitte Bressac–de Paillerets, Florence Démenais, Marie‐Françoise Avril, Valérie Chaudru, Patricia Jeannin, Fabienne Lesueur, E. Maubec, Hamida Mohamdi, M.J. Bossard, Amaury Vaysse, F. Boitier, Olivier Caron, F. Caux, Stéphane Dalle, O. Dereure, Dominique Leroux, Ludovic Martin, Christine Matéus, Caroline Robert, Dominique Stoppa‐Lyonnet, L. Thomas, E. Wierzbicka, David E. Elder, Michael E. Ming, Nandita Mitra, Tadeusz Dębniak, Jan Lubiński, Marko Hočevar, Srdjan Novaković, Barbara Perić, Petra Škerl, Johan Hansson, Veronica Höiom, Eitan Freidman, Esther Azizi, Orna Baron‐Epel, Alon Scope, Felix Pavlotsky, Irit Cohen‐Manheim, Yael Laitman, Mark Harland, Juliette A. Randerson‐Moor, Jon Laye, John Dwyfor Davies, Jérémie Nsengimana, Sally J. O’Shea, May Chan, Jo Gascoyne, Margaret A. Tucker, Alisa M. Goldstein, Xiaohong R. Yang, Rachael S. Stolzenberg-Solomon, Peter Kraft, Brian M. Wolpin, Pascal W.T.C. Jansen, Katherine A. McGlynn, Peter A. Kanetsky, Nilanjan Chatterjee, Jennifer H. Barrett, Alison M. Dunning, John Taylor, Julia Newton‐Bishop, D. Timothy Bishop, Þorkell Andrésson, Gloria M. Petersen, Christopher I. Amos, Mark M. Iles, Katherine L. Nathanson, Maria Teresa Landi, Michiel Vermeulen, Kevin M. Brown, Laufey T. Ámundadóttir

Bibliographic record

VenueNature Communications · 2017
Typearticle
Languageen
FieldMedicine
TopicTelomeres, Telomerase, and Senescence
Canadian institutionsPublic Health OntarioUniversity of TorontoLunenfeld-Tanenbaum Research InstituteCancer Care OntarioMount Sinai Hospital
FundersDivision of Cancer Epidemiology and Genetics, National Cancer InstituteMedical Research CouncilNederlandse Organisatie voor Wetenschappelijk OnderzoekNational Cancer InstituteNational Institutes of HealthU.S. Department of Health and Human ServicesCancer Research UKNational Institute for Health and Care ResearchFrederick National Laboratory for Cancer Research
KeywordsAlleleGenome-wide association studyBiologyGene knockdownLocus (genetics)TelomeraseTelomereSingle-nucleotide polymorphismGeneticsGenePancreatic cancerGene silencingGenotypeMolecular biologyCancer

Abstract

fetched live from OpenAlex

Genome wide association studies (GWAS) have mapped multiple independent cancer susceptibility loci to chr5p15.33. Here, we show that fine-mapping of pancreatic and testicular cancer GWAS within one of these loci (Region 2 in CLPTM1L) focuses the signal to nine highly correlated SNPs. Of these, rs36115365-C associated with increased pancreatic and testicular but decreased lung cancer and melanoma risk, and exhibited preferred protein-binding and enhanced regulatory activity. Transcriptional gene silencing of this regulatory element repressed TERT expression in an allele-specific manner. Proteomic analysis identifies allele-preferred binding of Zinc finger protein 148 (ZNF148) to rs36115365-C, further supported by binding of purified recombinant ZNF148. Knockdown of ZNF148 results in reduced TERT expression, telomerase activity and telomere length. Our results indicate that the association with chr5p15.33-Region 2 may be explained by rs36115365, a variant influencing TERT expression via ZNF148 in a manner consistent with elevated TERT in carriers of the C allele.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.037
GPT teacher head0.331
Teacher spread0.294 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations54
Published2017
Admission routes1
Has abstractyes

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Same venueNature CommunicationsSame topicTelomeres, Telomerase, and SenescenceFrench-language works237,207