Novel Plant Regeneration and Transient Gene Expression in Catharanthus roseus
Bibliographic record
Abstract
Catharanthus roseus genetic transformation represents a real challenge due, in part, to the lack of regeneration capability and this species’ recalcitrance to genetic transformation. In the present work, we demonstrate the regeneration of C. roseus plants from hypocotyls and cotyledons, using specific growth regulator conditions. Plants derived from hypocotyls and cotyledons were successfully acclimated and grown in the greenhouse. Furthermore, C. roseus meristem tissues were shown to have high shoot regeneration potential under conditions optimised for cotyledon- and hypocotyl-derived shooting. Meristem tissues were therefore investigated as a genetic transformation targets using both Agrobacterium tumefaciens and A. rhizogenes. Although meristem-derived shoots transformed with A. tumefaciens harbouring a p35S GUSplus construct revealed transient GUS expression and protein accumulation, they were not amenable to selection even after two months on selection medium. Transformation of C. roseus meristem tissues with A. rhizogenes resulted in a typical hairy root phenotype, in which the adventitious root tissue strongly expressed the p35S GUSplus construct, as revealed by intense GUS staining.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".