Development and application of Apx II-ELISA diagnostic kit for Actinobacillus pleuropneumoniae.
Bibliographic record
Abstract
According to the characterization of Actinobacillus pleuropneumoniae(APP) ApxⅡ,we developed a diagnostic kit,whose specificity,sensitivity,repeatability and stability were evaluated and compared with the indirect haemagglutination assay(IHA).A total of 1 453 clinical sera from China,UK,Denmark,USA,Canada and Australia were screened using our ApxⅡ-ELISA kit.The results showed that the ApxⅡ-ELISA kit has very high specificity、sensitivity and repeatability(the inter-and intrabatch imprecision(CV%) 15%) and stability(very stable at 4-8℃ for 9 months).Compared with the indirect haemagglutination assay(IHA),ApxⅡ-ELISA is more sensitive than IHA.Our data confirmed that the ApxⅡ-ELISA kit can be used to detect the antibodies of the toxin ApxⅡ and evaluate the effect of App vaccine.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".