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Record W2611289720 · doi:10.1073/pnas.1703498114

Maize <i>defective kernel</i> mutant generated by insertion of a <i>Ds</i> element in a gene encoding a highly conserved TTI2 cochaperone

2017· article· en· W2611289720 on OpenAlexaff
Nelson Garcia, Yubin Li, Hugo K. Dooner, Joachim Messing

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2017
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicChromosomal and Genetic Variations
Canadian institutionsBiotechnology Research Institute
FundersNational Science Foundation
KeywordsTransposable elementGeneGeneticsBiologyMutantComplementationHomology (biology)Transformation (genetics)Computational biology

Abstract

fetched live from OpenAlex

Significance Transposable elements (TEs) are important tools to study gene function in plants, more so than in animal species, because of the ease in generating large numbers of germinal transpositions. We took advantage of an engineered TE in maize to broaden this approach. We screened for a defective kernel mutant and used the green fluorescent protein encoded by the TE to locate the gene linked to the mutation. The property of the TE permitted us to generate revertants and new alleles of the tagged gene, avoiding complementation assays made cumbersome by difficult transformation protocols in maize. Based on its sequence homology to a cochaperone, the tagged gene opens a line of research on the role of cochaperones in seed development.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.252
Threshold uncertainty score0.288

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.037
GPT teacher head0.264
Teacher spread0.227 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations30
Published2017
Admission routes1
Has abstractyes

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