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Record W2622121859 · doi:10.1166/jnn.2017.13710

The Importance of Calcium Ions in Poly-A RNA Mediated Tobacco Mosaic Virus-Like Rod Formation

2017· article· en· W2622121859 on OpenAlexafffund
Joshua G Lucate, Amy Szuchmacher Blum

Bibliographic record

VenueJournal of Nanoscience and Nanotechnology · 2017
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Virus Research Studies
Canadian institutionsMcGill University
FundersNatural Sciences and Engineering Research Council of CanadaFonds Québécois de la Recherche sur la Nature et les Technologies
KeywordsTobacco mosaic virusRNACoat proteinMaterials scienceRodBiophysicsCalciumIonVirusNanotechnologyBiologyBiochemistryVirologyChemistryOrganic chemistryMedicine

Abstract

fetched live from OpenAlex

Due to its well-understood structural and chemical features, Tobacco mosaic virus (TMV) has attracted interest as a template for producing a variety of novel nanostructured materials and devices. However, for TMV based nanotechnology to reach its potential, controlling the size of TMV rods without using infectious native RNA is a key issue. Here, we re-examine the interactions of TMV coat protein (CP) with poly A RNA. Our results confirm that poly A RNA can induce virus-like rod formation through interactions with the coat protein. In addition, we show that calcium ions have an important role in stabilizing such rods, a previously unknown interaction that enhances the reliability of poly A induced assembly.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.392
Threshold uncertainty score0.507

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.001
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.286
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2017
Admission routes2
Has abstractyes

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