Abstract WP112: Trans-arterial Regional Hypothermia Provides Robust Neuroprotection in Rat Permanent Middle Cerebral Artery Occlusion Model
Bibliographic record
Abstract
Background and Purpose: We previously reported the neuroprotective effects of trans-arterial regional hypothermia in rat transient middle cerebral artery occlusion (tMCAO) model (ISC 2014, ISC 2015). The tMCAO model is representative of the clinical setting of successful recanalization in acute ischemic stroke. However, it is not always possible to achieve successful recanalization. Herein, we investigated the neuroprotective effects of trans-arterial regional hypothermia in permanent MCAO (pMCAO) model as well. Methods: Three groups were provided to investigate the neuroprotective effects. Regional hypothermia group had a single infusion of 20ml/kg 10°C cold saline via the ipsilateral internal carotid artery for 15 min at 0 hr or 1 hr after the onset of pMCAO. Vehicle group had a 37°C warm saline infusion in the same manner. Control group had no infusion after pMCAO. The rats were tested for neurological score after 48-hour pMCAO and then sacrificed to evaluate infarction size and pathological condition. Immunohistochemical analysis was performed to investigate the neuronal apoptosis (NeuN, cleaved caspase 3), glial reaction (GFAP), and microglial activation (Iba-1). Results: The regional hypothermia group showed significantly better neurological score compared with the other groups (p<0.01). Infarct volume was significantly smaller in the regional hypothermia group (8.1±4.7% at 0 hr and 15.7±5.9% at 1 hr) than in the vehicle group (35.8±5.6% at 0 hr and 34.0±3.6% at 1 hr) and also in the control group (35.1±8.9%) (p<0.01). Neuronal apoptosis (p<0.01), reactive gliosis (p<0.01), and microglial activation (p<0.05) in the peri-infarct area were significantly inhibited in the regional hypothermia group compared with the other groups. The degree of the inhibition was somewhat more evident at 0 hr than at 1 hr. Conclusions: Our result demonstrated that trans-arterial regional hypothermia provided robust neuroprotection in pMCAO model as well as tMCAO model, suggesting that this therapy may have a wide clinical application in ischemic stroke treatment.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".