Fixation of Juvenile Cambium from Two Coniferous Species for Ultrastructural Study
Bibliographic record
Abstract
Samples of dormant and of actively growing cambial-zone tissue collected in June and March, respectively, from plantations of young white spruce (Picea glauca [Moench] Voss) and red pine (Pinus resinosa Ait.) near Ottawa, Canada, were fixed in five different solutions at three temperatures. Fixation quality was evaluated by electron microscopy.Not all cellular organelles were preserved equally well by the same fixative in the active and in the dormant conditions, or in both spruce and pine. In general, our best results were obtained with Karnovsky's fixative solution. Somewhat less satisfactory results were obtained with a glutaraldehyde-acrolein mixture followed by straight glutaraldehyde. Poorer results were obtained with a trialdehyde solution while a glutaraldehyde-osmium tetroxide cocktail undoubtedly provided the worst fixation.Different fixative temperatures had a marked effect on fixation quality only when phosphate buffer was used. There was little gained by prolonging the period of fixation beyond a few hours.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".