Abstract 16: Adoptive Transfer of T Lymphocytes Prevents Angiotensin II-induced Vascular Stiffness in rag-1 Knockout Mice.
Bibliographic record
Abstract
Background: Both innate and adoptive immune systems are involved in the pathogenesis of hypertension and vascular damage. T lymphocytes participate in the low-grade inflammatory response that contributes to vascular injury in cardiovascular disease, including hypertension. Angiotensin (Ang) II-induced hypertension and endothelial dysfunction are blunted in rag1 knockout (rag1-/-) mice, which are deficient in T and B lymphocytes, and restored with adoptive transfer of T but not B lymphocytes. We hypothesized that adoptive transfer of T lymphocytes from C57Bl/6 mice (WT) will exacerbate Ang II-induced vascular damage in rag1-/- mice. METHODS: Eleven-week old male rag1-/- mice were injected i.v. with PBS or 10 x 106 pan T lymphocytes, and 2 weeks later implanted with a dummy pump (control) or infused with Ang II (490 ng/kg/min, s.c.) for 14 days (n=5-8). Systolic (SBP) and diastolic blood pressure (SBP) were measured by telemetry. Endothelial function and vessel structure were assessed in second order mesenteric arteries by pressurized myography. Successful adoptive transfer was confirmed at time of sacrifice by determining numbers of T cells in the spleen by flow cytometry. Results: Ang II induced a similar 40 mmHg SBP rise in rag-1-/- mice injected with PBS or T cells, but DBP rise was greater for T cell-injected mice (24 mmHg) than for PBS injected mice (9 mmHg). Ang II impaired vasodilatory responses to acetylcholine in T cell-injected mice (54.0±5.9%) but not in PBS injected mice (82.1±2.9%). Ang II treatment induced hypertrophic remodeling in PBS injected mice, but not in T cell-injected mice (media-to-lumen ratio: 4.1±0.3 from 2.7±0.3 vs. 3.3±0.2 from 2.6±0.1, media cross-sectional area (μm2): 5757±565 from 4382±580 vs. 4811±281 from 4350±254). Ang II increased vascular stiffness, indicated by a leftward shift of the small artery stress/strain relationship, in PBS injected mice but to a lower extent in T cell-injected mice. Conclusions: These findings suggest that T lymphocytes have a protective role in Ang II-induced vascular stiffness.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".