A Comparative Kinetic Study of Free and Immobilized Urease on Commercial and Glutaraldehyde Activated Cotton
Bibliographic record
Abstract
Urease was immobilized on commercial cotton and commercial cotton activated with glutaraldehyde. The kinetic of urea hydrolysis by free and immobilized urease was studied as a function of pH, temperature and time of hydrolysis. High concentration of ammonia was released at pH 10.0 for free enzyme (0.097 mg/mL) and urease immobilized on cotton (0.092 mg/mL) and pH 4.0 for enzyme, immobilized on activated commercial cotton (0.083 mg/mL). High concentration of ammonia was released at 30 °C for free enzyme (0.006 mg/mL), 25 °C for urease immobilized on cotton (0.043 mg/mL) and urease immobilized on cotton activated with glutaraldehyde (0.015 mg/mL). High concentration of ammonia was released after 10 minutes for free enzyme (0.0016 mg/mL), 60 minutes for inactivated (0.043 mg/mL) and for activated cotton (0.015 mg/ml). The result show that immobilized urease is less effective than the free enzyme and is more active in acid medium than the basic medium.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".