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The essential role of IL-7 signalling in CD8+ T-cell activity (57.17)

2011· article· en· W27009235 on OpenAlexaff
Angela M. Crawley, Agatha Vranjkovic, Mikki McGuinty, Elliott Faller, Paul MacPherson, Jonathan B. Angel

Bibliographic record

VenueThe Journal of Immunology · 2011
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsOttawa HospitalUniversity of Ottawa
Fundersnot available
KeywordsCell biologyT cellPerforinInterleukin-7 receptorCD8BiologyInterleukin 15PI3K/AKT/mTOR pathwaySignal transductionSTAT5IntracellularCell growthImmune systemInterleukinImmunologyCytokineIL-2 receptorBiochemistry

Abstract

fetched live from OpenAlex

Abstract Anti-viral CD8+ T-cell responses become impaired in HIV infection in part due to decreased T-cell survival, function and memory cell development; aspects mediated largely by interleukin-7 (IL-7). In progressive HIV infection, decreased T-cell expression of the IL-7 receptor α (CD127) and impaired IL-7 signalling, despite increased IL-7 production, may contribute to failing T-cell activity. Here we examine the effects of IL-7 on a panel of signalling pathways and investigates how IL-7 signalling pathways are associated with IL-7-related activities in CD8+ T-cells. Low concentrations of IL-7 (10 pg/ml) are capable of inducing maximum activation of the Jak-STAT and PI3K signalling pathways, while higher concentrations (500-1000 pg/ml) were required to induce Bcl-2 production and glucose uptake. Even higher concentrations of IL-7 (10,000 pg/ml) were needed to induce cell proliferation and perforin release. Inhibition of Jak activation reduced IL-7-induced Bcl-2, perforin production and proliferation. The activation of intracellular signalling pathways by IL-7 in human CD8+ T-cells and how they are associated with IL-7-induced functions has been extensively studied. Furthermore, the kinetic and optimal concentrations of IL-7 required for the induction of these functional outcomes suggest a complex control of IL-7-associated functions. This may provide insight in immune restoration therapies in HIV infection, where IL-7 signalling and functions are impaired.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.020

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0060.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.213
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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