Plant Cell Responses to Naphthenic Acids and Acid Extractable Organics Derived from Oil Sands Tailings Pond Water
Bibliographic record
Abstract
Mining of bitumen from the oil sands located in Alberta, Canada results in the production of oil sands process-affected water (OSPW). Naphthenic acids (NAs) are a constituent of the acid extractable organic (AEO) fraction isolated from OSPW. Detrimental phenotypic responses are apparent in plants exposed to NAs and AEO fraction. The aim of this thesis is to characterize the responses of plant cell structures after treatment with NAs and AEO fraction. Disruption to microtubules, actin filaments, mitochondria, peroxisomes, Golgi bodies, ER, and cell membranes was observed in onion epidermal cells and Arabidopsis thaliana root epidermal cells. Increasing reactive oxygen levels induced by NA and AEO exposure, and decreased cell viability after AEO exposure was also observed. This research represents the initial characterization of organelle and cytoskeletal responses after NA and AEO exposure in eukaryotic cells, and highlights the potential of cytoskeleton and organelle markers as a biosensor for OSPW toxicity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".