Connexin40 is required for NADPH oxidase‐derived oxidant production in microvascular endothelial cells
Bibliographic record
Abstract
The function of connexins (Cx) may extend beyond gap junctional intercellular communication (GJIC). We hypothesized that Cx40 is required for NADPH oxidase‐dependent ROS production in microvascular endothelial cells subjected to hypoxia/reoxygenation (H/R), independent of GJIC. ROS generation and NADPH oxidase activation was assessed after H/R in confluent monolayers of cells of wild‐type (WT), Cx40−/−, gp91phox−/−, or p47phox−/− mice, as well as in monolayers of GJIC‐deficient SKHep1 cells overexpressing GFP‐tagged Cx40. In WT cells, H/R‐initiated ROS increase was not affected by the GJ blocker 18alpha‐glycyrrhetinic acid, nor by preventing cells from establishing contact with each other, but it was absent in Cx40−/− cells. H/R increased ROS in SKHep1 cells expressing Cx40‐GFP, but not in cells expressing the control vector. We also asked if Cx40‐dependent reduction in inter‐endothelial electrical coupling after H/R requires NADPH oxidase. H/R reduced coupling in WT and gp91phox−/− but not in p47phox−/− cells. We suggest that (1) H/R‐initiated signaling in microvascular endothelial cells involves cross‐talk between NADPH oxidase and Cx40, and (2) Cx40 is required for NADPH oxidase‐derived ROS production, independent of GJIC. (Support: HSFO).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".